| FNDC1

FNDC1 (Fibronectin Type III Domain Containing 1) is a Protein Coding gene. Diseases associated with FNDC1 include Prostate Leiomyosarcoma and Prostate Sarcoma. An important paralog of this gene is ABI3BP.FNDC1 is a pathogenic gene in children with acute otitis media.Some studies found that knockdown AGS8 (also known as FNDC1) had a limited effect on epidermal growth factor and basic fibroblast growth factor signaling, indicating the specificity of AGS8-mediated trafficking. AGS8 is required for hypoxia-induced apoptosis of cardiomyocytes.FNDC1 can promote apoptosis in human salivary gland adenoid cystic carcinoma by hypermethylation.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.11	1.28	0.71	0.4	0.3	0.22	0.18	0.08
Corrected OD	2.03	1.2	0.63	0.32	0.22	0.14	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human FNDC1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-102	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human FNDC1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	85-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108